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Inzerční mutageneze ve funkční genomice Arabidopsis thaliana.

Basic information
Original title:Inzerční mutageneze ve funkční genomice Arabidopsis thaliana.
Authors:Jan Hejátko, Markéta Pernisová, Romana Dobešová, Jakub Horák, Břetislav Brzobohatý
Further information
Citation:HEJÁTKO, Jan, Markéta PERNISOVÁ, Romana DOBEŠOVÁ, Jakub HORÁK and Břetislav BRZOBOHATÝ. Inzerční mutageneze ve funkční genomice Arabidopsis thaliana. (Insertional mutagenesis in functional genomics of Arabidopsis thaliana.). In Kniha abstrakt, 3. metodické dny, Žďárske vrchy, Milovy. 2003.Export BibTeX
@proceedings{562649,
author = {Hejátko, Jan and Pernisová, Markéta and Dobešová, Romana and Horák, Jakub and Brzobohatý, Břetislav},
booktitle = {Kniha abstrakt, 3. metodické dny, Žďárske vrchy, Milovy},
keywords = {inzerční mutageneze; funkční genomika; reverzní genetika; Arabidopsis thaliana},
language = {eng},
title = {Inzerční mutageneze ve funkční genomice Arabidopsis thaliana.},
year = {2003}
}
Original language:English
Field:Genetics and molecular biology
Type:Conference abstract
Keywords:inzerční mutageneze; funkční genomika; reverzní genetika; Arabidopsis thaliana

To investigate the involvement of the putative sensor histidine kinase CKI1 in Arabidopsis thaliana development, we employed an approach that relies on the phenotype analysis of loss-of-function mutants. We report the identification of an allele of CKI1, cki1-i, which resulted from the insertion of an En-1 transposon into CKI1, and show that the mutation causes a block in embryo sac formation. We show that CKI1 is expressed throughout embryo sac development, and is switched on transiently during early seed development. These results demonstrate that gametophytic expression of a sensor-like molecule is essential for specific processes during megagametogenesis in Arabidopsis thaliana.

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