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Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings

Basic information
Original title:Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings
Authors:Petr Novák, Lukáš Žídek, Petr Padrta, Vladimír Sklenář
Information from University Press
Price:Not for sale
Publisher within MU:Faculty of Science
Further information
Citation:NOVÁK, Petr, Lukáš ŽÍDEK, Petr PADRTA and Vladimír SKLENÁŘ. Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings. In 19th NMR Valtice. Brno: Masarykova universita v Brně, 2004. p. 30-30, 1 pp. ISBN 80-210-3352-5.Export BibTeX
@inproceedings{571832,
author = {Novák, Petr and Žídek, Lukáš and Padrta, Petr and Sklenář, Vladimír},
address = {Brno},
booktitle = {19th NMR Valtice},
keywords = {NMR; Residual dipolar couplings; RNA; structure;},
language = {eng},
location = {Brno},
isbn = {80-210-3352-5},
pages = {30-30},
publisher = {Masarykova universita v Brně},
title = {Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings},
year = {2004}
}
Original language:English
Field:Biochemistry
Type:Article in Proceedings
Keywords:NMR; Residual dipolar couplings; RNA; structure;

The spliceosome is a complex of proteins and small nuclear RNAs (snRNAs) responsible for the removal of introns from pre-mRNA in eukaryotes. U6 snRNA has been demonstrated to be a vital element in splicing and its sequence is the most conserved of all snRNAs[1]. U6 snRNA from trypanosoma was our study. Determination of the solution structure of nucleic acid fragments is significantly improved if residual dipolar are measured. This data provides us additional long-range and local geometry restraints using a small degree of molecular alignment with the static magnetic field. We wanted to test polyethylenglycol (PEG) as a alignment medium for measuring the residual dipolar couplings (RDCs). PEG is a stable and unexpensive organic polymer that does not require a complex biochemical preparation like the most often used bacteriophage Pf1. In our study, applicability of PEG to several spin-state-selective E.COSY experiments was investigated. Measured RDCs were tested for internal consistency as refered in [2] in order to check the reliability of the values obtained in the PEG-aligned samples.

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