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Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specifity and kinetics

Basic information
Original title:Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specifity and kinetics
Authors:Ctirad Hofr, Pavla Šultesová, Michal Zimmermann, Iva Mozgová, Petra Procházková Schrumpfová, Michaela Wimmerová, Jiří Fajkus
Further information
Citation:HOFR, Ctirad, Pavla ŠULTESOVÁ, Michal ZIMMERMANN, Iva MOZGOVÁ, Petra PROCHÁZKOVÁ SCHRUMPFOVÁ, Michaela WIMMEROVÁ a Jiří FAJKUS. Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specifity and kinetics. BIOCHEMICAL JOURNAL, ENGLAND: PORTLAND PRESS LTD, 2009, roč. 419, Neuveden, s. 221-228. ISSN 0264-6021.Export BibTeX
@article{825540,
author = {Hofr, Ctirad and Šultesová, Pavla and Zimmermann, Michal and Mozgová, Iva and Procházková Schrumpfová, Petra and Wimmerová, Michaela and Fajkus, Jiří},
article_location = {ENGLAND},
article_number = {Neuveden},
keywords = {Fluorescence anisotropy; single-Myb-histone protein (SMH protein); surface plasmon resonance; telomere protein DNA interaction},
language = {eng},
issn = {0264-6021},
journal = {BIOCHEMICAL JOURNAL},
title = {Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specifity and kinetics},
volume = {419},
year = {2009}
}
Original language:English
Field:Genetics and molecular biology
Type:Article in Periodical
Keywords:Fluorescence anisotropy; single-Myb-histone protein (SMH protein); surface plasmon resonance; telomere protein DNA interaction

We performed the first quantitative DNA-binding study of the plant-specific family of SMH proteins. Interactions of full-length proteins AtTRB1 and AtTRB3 with telomeric DNA were analysed by electrophoretic mobility-shift assay, fluorescence anisotropy and surface plasmon resonance to reveal their binding stoichiometry and kinetics. Based on these data, a model explaining the binding stoichiometry and the protein arrangement on telomeric DNA is presented.

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