Monitoring of in vitro DNA synthesis and PCR amplification using 7-deaza-purines as electroactive markers
|Field:||Genetics and molecular biology|
|Type:||Article in Proceedings|
|Keywords:||7-deaza purines; electrochemical detection; carbon electrode; repetitive elements|
7 deaza purines (particularly 7 deaza guanine) incorporated into the DNA sequence during in vitro extension and/or PCR enable the simple electrochemical detection of the PCR products at the carbon eletrode. Based on the pilot experiments, the utilization of the method in a fast and simple analysis of G rich repetitive sequences in eukaryotic genomes is supposed.