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Monitoring of in vitro DNA synthesis and PCR amplification using 7-deaza-purines as electroactive markers

Basic information
Original title:Monitoring of in vitro DNA synthesis and PCR amplification using 7-deaza-purines as electroactive markers
Authors:Miloslava Fojtová, Petra Horáková, Hana Pivoňková, Eva Šimková, Zdenka Vychodilová, Luděk Havran, Miroslav Fojta
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Original language:English
Field:Genetics and molecular biology
Type:Article in Proceedings
Keywords:7-deaza purines; electrochemical detection; carbon electrode; repetitive elements

7 deaza purines (particularly 7 deaza guanine) incorporated into the DNA sequence during in vitro extension and/or PCR enable the simple electrochemical detection of the PCR products at the carbon eletrode. Based on the pilot experiments, the utilization of the method in a fast and simple analysis of G rich repetitive sequences in eukaryotic genomes is supposed.

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