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Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification

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Original title:Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification
Authors:Antonín Hlaváček, Pavel Bouchal, Petr Skládal
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Original language:English
Field:Biochemistry
Type:Article in Periodical
Keywords:biotin; avidin; albumin; bioconjugation

A competitive microplate fluoroimmunoassay was developed for the determination of human serum albumin in urine. It is based on the use of biotinylated CdTe quantum dots (QDs) whose synthesis is optimised in terms of storage stability, purification, and signal-to-noise ratio. The bioconjugated QDs were characterised by gel chromatography and gel electrophoresis. Storage stability and quantum yield were investigated. The excitation/emission wavelengths are 360/620 nm. The immunoassay of human serum albumin in urine has a working range from 1.7 to 10 ug/mL, and the limit of detection is 1.0 ug/mL.

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