Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols
|Original title:||Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols|
|Authors:||Petr Skládal, Eva Švábenská, Jiří Žeravík, Jan Přibyl, Petra Šišková, Torbjorn Tjarnhage, Inga Gustafson|
|Type:||Article in Periodical|
|Keywords:||Bioagents; Sandwich immunoassay; Biosensors; Remote sensing; Aerosol chamber|
The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5a in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108 CFU/mL within 30 min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150 CFU/L in air was clearly indicated (20 min analysis including 5 min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions.