Publication details

 

Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection.

Basic information
Original title:Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection.
Authors:Martina Lengerová, Iva Kocmanová, Zdeněk Ráčil, Kristýna Hrnčířová, Šárka Pospíšilová, Jiří Mayer, Laura K Najvar, Nathan P Wiederhold, William R Kirkpatrick, Thomas F Patterson
Further information
Citation:LENGEROVÁ, Martina, Iva KOCMANOVÁ, Zdeněk RÁČIL, Kristýna HRNČÍŘOVÁ, Šárka POSPÍŠILOVÁ, Jiří MAYER, Laura K NAJVAR, Nathan P WIEDERHOLD, William R KIRKPATRICK a Thomas F PATTERSON. Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection. Journal of Clinical Microbiology, Washington: American Society for Microbiology, 2012, roč. 50, č. 3, s. 602-608. ISSN 0095-1137. doi:10.1128/JCM.05356-11.Export BibTeX
@article{980422,
author = {Lengerová, Martina and Kocmanová, Iva and Ráčil, Zdeněk and Hrnčířová, Kristýna and Pospíšilová, Šárka and Mayer, Jiří and Najvar, Laura K and Wiederhold, Nathan P and Kirkpatrick, William R and Patterson, Thomas F},
article_location = {Washington},
article_number = {3},
doi = {http://dx.doi.org/10.1128/JCM.05356-11},
keywords = {Invasive pulmonary aspergillosis; real-time PCR; galactomannan; glucan},
language = {eng},
issn = {0095-1137},
journal = {Journal of Clinical Microbiology},
title = {Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection.},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22189110},
volume = {50},
year = {2012}
}
Original language:English
Field:Oncology and hematology
WWW:link to a new windowhttp://www.ncbi.nlm.nih.gov/pubmed/22189110
Type:Article in Periodical
Keywords:Invasive pulmonary aspergillosis; real-time PCR; galactomannan; glucan

We developed and assessed the diagnostic value of a novel quantitative nested real-time (QNRT) PCR assay targeting the internal transcribed spacer region of ribosomal DNA (rDNA) in a guinea pig model of invasive pulmonary aspergillosis. Groups of 5 immunosuppressed animals that were infected using an aerosol chamber with Aspergillus fumigatus conidia were humanely terminated 1 h postinoculation and at days 3,5,7,and 11 postchallenge, and lung tissue, bronchoalveolar lavage (BAL) fluid, whole blood, and serum samples were collected. The QNRT PCR results obtained with the serum and BAL fluid were compared to those achieved with galactomannan and (1,3)-beta-D-glucan assays. High fungal burden levels were detected by QNRT PCR in both lung tissue and BAL fluid in all infected animals at each time point, and the sensitivity of each assay in BAL fluid was 100% by day 3 and remained so through the remainder of the study. The sensitivity of detection of fungi in whole blood and serum samples was significantly lower, and some samples remained negative by all three assays despite the advanced stage of the infection. From these data, we can conclude that this novel QNRT PCR method was highly sensitive for the detection of A. fumigatus from different types of samples in this model. In addition, BAL fluid samples appeared to be the most suitable for the early diagnosis of invasive pulmonary aspergillosis. When testing serum, the use of a combination of available assays may increase the possibility of early detection of this opportunistic mycosis.

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