Voltammetric Study of dsDNA Modified by Multi-redox LabelBased on N-methyl-4-hydrazino-7-nitrobenzofurazan

• Authors: DAŇHEL Aleš; RAINDLOVA Veronika; HAVRAN Luděk; BAREK Jiří; HOCEK Michal; FOJTA Miroslav
• Year of publication: 2014
• Type: Article in Periodical
• Magazine / Source: Electrochimica Acta
• MU Faculty or unit: Central European Institute of Technology
• Web: http://www.sciencedirect.com/science/article/pii/S001346861400468X#
• Doi: http://dx.doi.org/10.1016/j.electacta.2014.02.137
• Field: Electrochemistry
• Keywords: DNA; Redox labeling; N-methyl-4-hydrazino-7-nitrobenzofurazan; Silver solid amalgam electrode; Voltammetry
• Description: Voltammetric behaviour of novel synthetic multi-redox DNA label N-methyl-4-hydrazino-7-nitrobenzofurazan (NBF), a deoxycytidine monophosphate conjugate with NBF via formylthiophenelinker (dCNBFMP), and NBF labeled model short double stranded DNA (dsDNA-NBF) were systematicallystudied at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) for the first time inthis work. Used modification resulted in increase of selectivity andsensitivity of the DNA voltammetric determination.

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