Publication details
The protein composition of excretory-secretory products of three Trichinella species
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| Year of publication | 2014 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The nematodes of the genus Trichinella are intracellular parasites of skeletal muscle cells. These organisms are able to parasitize carnivorous and omnivorous vertebrates, including man. They are causative agents of trichinellosis, an important worldwide zoonotic disease. Twelve Trichinella taxa are divided into two clades characterized by the presence or absence of an intramuscular collagen capsule. In the Czech Republic we recognize three trichinella species - Trichinella spiralis, T. britovi (mainly in carnivores and wild boars) and T. pseudospiralis (mainly in predatory birds). While T. spiralis and T. britovi induce the formation of a collagen capsule surrounding the parasite, T. pseudospiralis survives in a non-encapsulated form. Investigating the functions of various bioactive molecules produced by parasites expands our understanding to the principles of host-parasite interaction. We used High-Performance Liquid Chromatography (UltiMate® 3000 RSLCnano LC) in combination with Mass Spectrometry (Orbitrap-Elite MS system) and transcriptomic analysis (Illumina MiSeq) to reveal and compare the spectrum of molecules in excretory-secretory products and all transcripts of L1 larvae of these model organisms. Several of important functional protein compounds were observed and their key role in host-parasite cross-talking was estimated. 364 proteins in ES products of Trichinella spp. L1 larvae were identified, e.g. antigens - 53 kDa protein, 43 kDa glycoprotein or biologically active proteins – cysteine peptidases and their inhibitors. Some of these molecules were prepared in recombinant form. |