Publication details

Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro

Authors

RADASZKIEWICZ Katarzyna Anna SÝKOROVÁ Dominika KARAS Pavel KUDOVÁ Jana KOHÚT Lukáš BINÓ Lucia VEČEŘA Josef VÍTEČEK Jan KUBALA Lukáš PACHERNÍK Jiří

Year of publication 2016
Type Article in Periodical
Magazine / Source Review of Scientific Instruments
MU Faculty or unit

Faculty of Science

Citation
Web http://scitation.aip.org/docserver/fulltext/aip/journal/rsi/87/2/1.4941776.pdf?expires=1457558113&id=id&accname=guest&checksum=4E6E84A242B8B6216A78B92ACD4D6AE6
Doi http://dx.doi.org/10.1063/1.4941776
Field Physiology
Keywords cardiomyocytes; digital video; beating
Description The analysis of digital video output enables the non-invasive screening of various active biological processes. For the monitoring and computing of the beating parameters of cardiomyocytes in vitro, CB Analyser (cardiomyocyte beating analyser) software was developed. This software is based on image analysis of the video recording of beating cardiomyocytes. CB Analyser was tested using cardiomyocytes derived from mouse embryonic stem cells at different stages of cardiomyogenesis. We observed that during differentiation (from day 18), the beat peak width decreased, which corresponded to the increased speed of an individual pulse. However, the beating frequency did not change. Further, the effects of epinephrine modulating mature cardiomyocyte functions were tested to validate the CB Analyser analysis. In conclusion, data show that CB Analyser is a useful tool for evaluating the functions of both developing and mature cardiomyocytes under various conditions in vitro

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