Publication details
Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae): insight into composition of E/S products and basic characterization of serpin
| Authors | |
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| Year of publication | 2016 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | Two genes coding serpins, were expressed in E. coli system (BL21 strain) in combination with synthetic genes, optimised for this system, using pET-22B(+) expression vector. IPTG induced expression (30°C, overnight) was analyzed on SDS-PAGE and soluble recombinant serpins of E. nipponicum was partially purified using Ni-NTA agarose beads. Successful production of serpins was verified by mass spectrometry and Western blot using His-tag primary antibodies. |
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