Publication details

A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations

Authors

ABDEL-MOHSEN A.M. JANČÁŘ Josef ABDEL-RAHMAN R.M. VOJTEK Libor HYRŠL Pavel DUŠKOVÁ Monika NEJEZCHLEBOVÁ Helena

Year of publication 2017
Type Article in Periodical
Magazine / Source International Journal of Pharmaceutics
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1016/j.ijpharm.2017.02.003
Field Immunology
Keywords In-situ silver; hyaluronan; cytotoxicity; wound dressing
Description In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time. Different concentrations of silver nitrate (1, 2 mg/100 ml) were added at ambient temperature to sodium hyaluronate solution (40 mg/ml), then the pH was increased to 8 by adding sodium hydroxide. The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST). UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively. The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA 3 fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25 ± 2, nm size (TEM, DLS) and acceptable mechanical properties. The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability. The antibacterial performance of medical HA/Ag-NPs fabrics were evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity. The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT). In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process. Based on our results, the new HA/Ag-NPs-2 mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.

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