Publication details
Odezva izolovaných chloroplastů Lemna minor (L.) na diklofenak
| Title in English | Response of isolated chloroplasts of Lemna minor to diclofenac |
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| Authors | |
| Year of publication | 2017 |
| Type | Article in Proceedings |
| Conference | Vliv abiotických a biotických stresorů na vlastnosti rostlin 2017. Recenzovaný Sborník |
| MU Faculty or unit | |
| Citation | |
| Field | Physiology |
| Keywords | Lemna minor; diclofenac; suspension of chloroplasts; Hill reaction activity; chlorophyll fluorescence; |
| Description | Results coming from the assessment of the effect of increasing concentration of diclofenac (1, 10, 100 a 1000 uM) on suspension of isolated duckweed (Lemna minor) chloroplasts proved a decrease in values of potential and effective quantum yields of chlorophyll fluorescence and non-photochemical quenching (Fv/Fm by 27%, FII by 44% qN, by 6% compared to control) and increase in basal fluorescence level (Fo, by 2% compared to control) only when exposed to DCF 1000 uM. Hill reaction activity of isolated duckweed chloroplasts was significantly inhibited only at DCF 100 uM and especially 1000 uM. The results show, that a higher then environment treatment of diclofenac simulating chronic (or high acute) effects may have a negative impact on biochemical and physiological processes at Lemna minor. They also illustrate the need for multi-level drug testing, because of lower sensitivity of standardized ecotoxicity tests. |
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