Publication details
Biochemical and cytological changes of tobacco BY-2 cells under diclofenac impact
| Authors | |
|---|---|
| Year of publication | 2019 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Studies focusing on environmental contamination have shown that besides “classic” persistent pollutants, pseudo-persistent emerging contaminants are also present. As highly specific biologically active substances, pharmaceuticals and pharmaceutically active substances entering the environment can influence non-target organisms including plants. One of the commonly detected and monitored pharmaceuticals in water is a non-steroidal anti-inflammatory drug diclofenac (DCF). Plant cell suspension cultures like Nicotiana tabacum BY-2 can be a suitable model for assessing toxicity of wide range of inorganic and organic pollutants at the cellular and subcellular levels. The question is, if suspension BY-2 can be used even for drug toxicity evaluation. Results of this study suggest that exposure to environmentally relevant concentrations of DFC (1, 10, and 100 ug/L) affected significantly the viability of BY-2 cells, mitotic index, suspension density and dry weight. DCF-induced oxidative stress documented by increased production of total reactive oxygen species (ROS) triggered apoptotic alterations in BY-2 cells leading to programmed cell death – occurrence of acidic compartments and autophagic vacuoles in BY-2 cells and apoptotic chromatin condensation and DNA fragmentation in their nuclei. Due to its prompt and sensitive response tobacco BY-2 cell suspension is suitable for evaluation of phytotoxicity of drugs. |
| Related projects: |