Publication details
Intrinsic atomic interaction at molecular proximal vicinity infer cellular biocompatibility of antibacterial nanopepper
| Authors | |
|---|---|
| Year of publication | 2021 |
| Type | Article in Periodical |
| Magazine / Source | NANOMEDICINE |
| MU Faculty or unit | |
| Citation | |
| Web | https://www.futuremedicine.com/doi/10.2217/nnm-2020-0395 |
| Doi | http://dx.doi.org/10.2217/nnm-2020-0395 |
| Keywords | antibacterial; black pepper; caspase; cytotoxicity; HCT116 cells; piperine; Sod1 |
| Description | Aim: Fabrication of nanopepper (NP) for antibacterial application and elucidation of its molecular and cellular biocompatibility. Materials & methods: Synthesis of NP was achieved using a high-energy ball milling method. Following characterization, its antibacterial activity and cellular and molecular biocompatibility were evaluated in vitro by experimental and computational approaches. Results: A total of 15 h of milling pepper produced NP with a size of 44 +/- 12 nm and zeta potential of -22 +/- 12 mV. Bulk pepper and NP showed antibacterial activity and an LC50 of 1.9 mu M and 2.1 mu M in HCT116 colon cells. Components of pepper, piperine and beta-caryophyllene were found to interact with superoxide dismutase [Cu-Zn] and apoptotic protease-activating factor-1-caspase-9 through different amino acids via H-bonds. Conclusion: NP exhibits significant antibacterial activity with cellular biocompatibility due to intrinsic atomic interaction. |