Publication details
Sledování stability transgenu u Drosophila melanogaster pomocí teplotní gredientové gelové elektroforézy a sekvencování
| Title in English | Stability of trangene in Drosophila melanogaster studiyng by temperature gradient gel electrophoresis and sequencing |
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| Authors | |
| Year of publication | 2001 |
| Type | Article in Proceedings |
| Conference | V. Pracovní setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | transgenic Drosophila melanogaster; DGGE |
| Description | The fly Drosophila melanogaster is one of the most intensively studied organisms in biology. The relatively small genome makes it a streamlined animal genome ideal for genetic analysis. The Drosophila genome is ~180 Mb in size and encodes ~13.600 genes. It has four chromosomes. We are interested in analysing stability of a transgenes which were inserted to Drosophila genome by P elements transposition. Temperature Gradient Gel Electrophoresis (TGGE) is a new and powerful electrophoresis method for separation of nucleic acids or for analysis of proteins. TGGE system, compare with standard electrophoresis methods, adds an additional parameter for separation, namely the conformation (shape) of molecules. The shape is mostly determined by the secondary and tertiary structure of the molecule and can be changed by external influences like temperature, salt concentration, pH etc. TGGE uses Tm to identify DNA which differs in a sequence among a mixture or molecules of the same size. |
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