Elicitation of tobacco cells with ergosterol activates a signal pathway including mobilisation of internal calcium

• Authors: KAŠPAROVSKÝ Tomáš; MILAT ML; MIKEŠ Vladimír; HAVEL Ladislav
• Year of publication: 2003
• Type: Article in Periodical
• Magazine / Source: Plant Physiol Biochem
• MU Faculty or unit: Faculty of Science
• Field: Biochemistry
• Keywords: ergosterol; calcium channels; elicitor; Phytophthora; tobacco
• Description: Ergosterol interacts with tobacco suspension (Nicotiana tabacum) cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. The use of several inhibitors of calcium channels (La3+, TMB-8, verapamil, ruthenium red, nifedipine) and a protein-kinase inhibitor (staurosporin) suggests that the elicitation with ergosterol includes the mobilization of internal calcium stores mediated by inositol 1,4,5-trisphosphate and serine/threonine protein kinases.

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