Electrophoretically mediated microanalysis (Review)

• Authors: VYTISKOVÁ Soňa; VAN DYCK Sigrid; VAN SCHEPDAEL Ann; HOOGMARTENS Jos; GLATZ Zdeněk
• Year of publication: 2004
• Type: Article in Periodical
• Magazine / Source: Journal of Chromatography A
• MU Faculty or unit: Faculty of Science
• Field: Biochemistry
• Keywords: Capillary electrophoresis; enzymes; EMMA
• Description: This review describes the existing developments in the use of the capillary electrophoretic microanalytical technique for the in-line study of enzyme reaction, electrophoretically mediated microanalysis (EMMA). The article is divided into a number of parts. After an introduction, the different modes, basic principle, procedure, and some mathematical treatments of EMMA methodology are discussed and illustrated. The applications of EMMA for enzyme assay and for non-enzymatic determination are summarized into two tables. In addition to classical capillary electrophoresis (CE) instrument EMMA, special emphasis is given to a relatively new technique: EMMA on CE microchip. Finally, conclusions are drawn.

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