Publication details
Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection.
| Authors | |
|---|---|
| Year of publication | 2004 |
| Type | Article in Periodical |
| Magazine / Source | Analytica Chimica Acta |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | diode array detection; drugs; fat-soluble vitamins; high-performance liquid chromatography; mass spectrometry; pharmaceutical preparations; simultaneous determination; vitamins; water-soluble vitamins |
| Description | Water- and fat-soluble vitamins were separated on a MetaChem Polaris C18-A (150 mm x 4.6 mm, 3 mum particle size) in a single run using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010% trifluoroacetic acid of pH 3.9 (solvent A) and methanol (solvent B) at the flow rate 0.7 ml min(-1). A linear gradient profile (A:B) started at 95:5 and was constant in the first 4 min, then linearly decreased up to 2:98 during the next 6 min, then it was constant in the next 20 min and finally linearly increased up to 95:5 ratio of water phase in the last 5 min of the separation. The most suitable detection wavelength for simultaneous vitamin determination was 280 nm. The method was applied for the solid sample of pharmaceutical preparation (B-Komplex), fortified powdered drinks (multi-vitamin) and food samples. The results were in good agreement with the declared values. |