Publication details
Multichannel SPR biosensor for detection of endocrine-disrupting compounds
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | Analytical and Bioanalytical Chemistry |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.springerlink.com/content/y04j275j15246x12/fulltext.html |
| Field | Biochemistry |
| Keywords | Surface plasmon resonance; Nonylphenol; Atrazine |
| Description | A surface plasmon resonance (SPR) biosensor for simultaneous detection of multiple organic pollutants exhibiting endocrine disrupting activity, namely atrazine, benzo[a]pyrene, 2,4dichlorophenoxyacetic acid (2,4D) and nonylphenol, is reported. The biosensor utilizes a multichannel SPR sensor based on wavelength modulation of SPR and wavelength division multiplexing (WDM) of sensing channels, antibodies as biorecognition element and a competitive immunoassay detection format. An analysis time of 45 min (including 30 min incubation of the sample with antibodies) and limits of detection as low as 0.05, 0.07, 0.16 and 0.26 ng/mL are demonstrated for benzo[a]pyrene, atrazine, 2,4D and nonylphenol, respectively. The biosensor is also shown to be regenerable and suitable for repeated use |
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