Identification of antifungal proteins in the barley and malt caryopsis by mass spectrometry

• Authors: HÉGROVÁ Blanka; MACUCHOVÁ Simona; PREISLER Jan; HAVEL Josef
• Year of publication: 2008
• Type: Article in Proceedings
• Conference: Vitamins 2008 Nutrition and Diagnostics
• MU Faculty or unit: Faculty of Science
• Field: Analytic chemistry
• Keywords: low molecular weight proteins; barley; malt; SDS-PAGE; enzymatic digestion; MALDI-TOF mass spectrometry
• Description: Recently, great attention is paid to determination of various metabolites after pathogen-caryopsis interaction. Some of antifungal proteins, e.g. thionins, might be of effect on barley quality and its subsequent food products - gushing or premature yeast flocculation. The changes proceeding during pathogen-caryopsis interaction were monitored in 20 samples of barley and its corresponding malt by the combination of Sodium Dodecyl Sulphate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Matrix-Assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF MS).

Related projects

• Study of the chemical changes of barley caryopses after an attack by pathogens to monitor and improve the quality of barley and its derived food products