Publication details
Quantitative proteomic analysis of lung carcinoma cell line transfected by mutant p53 gene sequences
| Authors | |
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| Year of publication | 2009 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The present study constitutes the use of a quantitative LC-MS/MS based proteomic approach for the comparative proteomic analysis of lung carcinoma cell line H1299 steady transfected by two different mutant p53 gene sequences (hot-spot mutations at codon positions 175 and 273, respectively). The proteomics analysis method involved iTRAQ 8-plex stable isotope labeling of tryptic peptides, their separation via off-line coupled two-dimensional liquid chromatography (SCX and RP-LC) followed by tandem mass spectrometry on the MALDI-TOF/TOF platform (iTRAQ-2DLC-MS/MS). The principal aims of this study were: (1) to define the protein spectrum detectable using this approach, and (2) to quantitative compare the proteomic profiles of cells, which differ only in type of p53 mutation after 17-(allylamino)-17-demethoxygeldanamycin (17-AAG) treatment. The study resulted in the reproducible identification of 411 non-redundant proteins. |
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