Publication details

Biosensor for determination of carboxylic acids in wines based on the inhibition of sarcosine oxidase

Authors

ŽERAVÍK Jiří LACINA Karel JÍLEK Milan VLČEK Jiří SKLÁDAL Petr

Year of publication 2010
Type Article in Periodical
Magazine / Source Microchimica Acta
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords Biosensor; Sarcosine oxidase; Carboxylic acid; Wine;
Description The flow-through amperometric biosensor is presented for determination of carboxylic acids. It is based on two sensor layers that are deposited on a platinum electrode. The inner layer serves to eliminate interferences by limiting diffusion of electrochemically active substances such as ascorbic acid. This layer is electro-polymerized using an equimolar mixture of o-phenylenediamine and resorcinol. The outer layer is prepared by cross-linking the enzyme sarcosine oxidase and bovine serum albumin using glutaraldehyde. The formation of enzymatically produced hydrogen peroxide is monitored at 600 mV vs. an Ag/AgCl reference electrode. The addition of carboxylic acids causes competitive inhibition of the enzyme and a decrease in signal. The assay was optimized for determination of carboxylic acids in wine samples. Following 10-fold dilution, most samples contain 1-10 mM individual carboxylic acids and thus a 5 mM concentration of sarcosine was chosen as being optimal for competition.
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