Publication details
Elicitory vyvolaná obranná reakce v suspenzních buněčných kulturách tabáku
| Title in English | Defence reaction induced by elicitors in tobacco cell suspension cultures |
|---|---|
| Authors | |
| Year of publication | 2010 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Cryptogein is an elicitin secreted by Phytophtora cryptogea. Cryptogein binds sterols and fatty acids and this binding evokes a structural shift of omega-loop region. Cryptogein mutants with altered ability to bind sterols and fatty acids were prepared. Hydrogen peroxide, glutathione and nitric oxide were determined in tobacco (Nicotiana tabacum) cell suspension cultures treated with cryptogein and its mutants. As previously reported, cryptogein triggered a rapid NO and H2O2 accumulation and a decrease of reduced glutathione. In contrast, the responses of tobacco cells induced by cryptogein mutants differed considerably. Cryptogein mutants with reduced ability to bind sterols or to fatty acids induced slower or transient reactions. |