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HPLC-ECD determination of hydroxyl radicals in various experimental models

Basic information
Original title:HPLC-ECD determination of hydroxyl radicals in various experimental models
Authors:Hana Paulová, Hana Bochořáková, Marie Nováková, Eva Táborská
Further information
Citation:PAULOVÁ, Hana, Hana BOCHOŘÁKOVÁ, Marie NOVÁKOVÁ and Eva TÁBORSKÁ. HPLC-ECD determination of hydroxyl radicals in various experimental models (HPLC-ECD determination of hydroxyl radicals in various experimental models). In In VITAMINS, NUTRITION, DIAGNOSTICS 2009. 2009. ISBN 978-80-7318-809-2.Export BibTeX
@proceedings{861344,
author = {Paulová, Hana and Bochořáková, Hana and Nováková, Marie and Táborská, Eva},
booktitle = {In VITAMINS, NUTRITION, DIAGNOSTICS 2009},
keywords = {HPLC; coulometric detection; hydroxyl radical},
language = {eng},
isbn = {978-80-7318-809-2},
title = {HPLC-ECD determination of hydroxyl radicals in various experimental models},
year = {2009}
}
Original language:English
Field:Biochemistry
Type:Conference abstract
Keywords:HPLC; coulometric detection; hydroxyl radical

The production of hydroxyl radicals was evaluated as products of salicylic acid hydroxylation using HPLC with coulochemical detector. Two models were used. In human studies we evaluated the production of hydroxyl radicals in patients with cardiovascular disease and in healthy volunteers. The determination of hydroxyl radicals in vivo was based on the evaluation of hydroxylation products of salicylic acid after the ingestion of acetylsalicylic acid (Aspirin). In animal studies we measured the production of hydroxyl radicals in isolated rat and guinea pig hearts during ischemia-reperfusion injury. Isolated hearts were perfused with Krebs-Henseleit buffer containing 1mM salicylic acid according to the Langendorff method. The both methods above illustrate a wide field of efficiency of HPLC methods coupled with coulometric detection for detection of the parameters of oxidative stress.

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