Publication details
Proximal and distal nerve injuries induce changes of endoneurial laminin
-1 in the spinal nerve roots - A quantitative immunohistochemical assessment
| Basic information | |
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| Original title: | Proximal and distal nerve injuries induce changes of endoneurial laminin-1 in the spinal nerve roots - A quantitative immunohistochemical assessment |
| Authors: | Marek David, Petr Dubový, Ivana Hradilová Svíženská, Ilona Klusáková |
| Further information | |
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| Citation: | DAVID, Marek, Petr DUBOVÝ, Ivana HRADILOVÁ SVÍŽENSKÁ and Ilona
KLUSÁKOVÁ. Proximal and distal nerve injuries induce changes of
endoneurial laminin-1 in the spinal nerve roots - A
quantitative immunohistochemical assessment (Proximal and
distal nerve injuries induce changes of endoneurial laminin-1
in the spinal nerve roots - A quantitative immunohistochemical
assessment). In Joint Conference of the Czech and Slovak
Neuroscience Societies. 2009.Export BibTeX @proceedings{854486, author = {David, Marek and Dubový, Petr and Hradilová Svíženská, Ivana and Klusáková, Ilona}, booktitle = {Joint Conference of the Czech and Slovak Neuroscience Societies}, keywords = {behavioral tests; bilateral reaction of spinal nerve roots; dorsal root; endoneurial laminin -1; neuropathic pain; peripheral nervous system; proximal and distal injuries of sciatic nerve; ventral root},language = {eng}, title = {Proximal and distal nerve injuries induce changes of endoneurial laminin -1 in the spinal nerve roots - A quantitative immunohistochemical assessment},url = {http://www.biomed.cas.cz/uem/neuro2009/}, year = {2009} } |
| Original language: | English |
| Field: | Neurology, neurosurgery, neurosciences |
| WWW: |  web stránky konference |
| Type: | Conference abstract |
| Keywords: | behavioral tests; bilateral reaction of spinal nerve roots; dorsal root; endoneurial laminin-1; neuropathic pain; peripheral nervous system; proximal and distal injuries of sciatic nerve; ventral root |
Events in the spinal nerve roots after distal peripheral nerve injury, which induces neuropathic pain, are little characterized. Our previous results from immunofluorescence (IF) microscopy and computer-assisted image analysis revealed endoneurial extracellular matrix remodulation following dorsal and ventral rhizotomy. Therefore, we performed a quantitative immunohistochemical analysis to detect changes of laminin-1 (lam-1) in the ipsi- and contralateral spinal nerve roots of two neuropathic pain models - sciatic (ScNL) and spinal nerve (SNL) ligature. Behavioral tests demonstrated neuropathic pain induction (mechanical allodynia and thermal hyperalgesia) in ipsilateral hind paws of all experimental animals. A higher IF intensity of lam-1 was found in the ventral compared to dorsal roots of naive rats. In contrast to naive rats, significant bilateral enhancement of lam-1 IF was detected in the dorsal and ventral roots 1 to 3 days after ScNL as well as 1 to 7 days after SNL. Endoneurial lam-1 IF intensity in both ipsi- and contralateral spinal nerve roots was decreased 7 days following ScNL and 14 days from SNL. The decreased lam-1 IF intensity in the endoneurium of spinal nerve roots was to the level of IF brightness measured in naive animals. Even if endoneurial lam-1 IF changes are more distinct on ipsi- than contralateral side, their patterns in contralateral spinal nerve roots coincided with ipsilateral ones after both types of unilateral nerve ligature. Generally, lam-1 IF changes are larger in ScNL than SNL model of neuropathic pain. In conclusion, both proximal and distal nerve injuries lead to remodulation of the endoneurial lam-1 in the spinal nerve roots.
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