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HPLC method for analysis of selected groups of isoquinoline alkalois

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Original title:HPLC method for analysis of selected groups of isoquinoline alkalois
Authors:Jana Suchomelová, Eva Táborská, Hana Bochořáková
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Original language:English
Field:Analytic chemistry
Type:Article in Proceedings
Keywords:alkaloids; isoquinoline; HPLC; benzophenanthridine; protopine; protoberberine;

Three types of HPLC reversed phase columns: SupelcosilTM LC-18, Synergi MAX RP C12, Synergi Fusion RP C18 were used for HPLC resolution of five protopine, nine protoberberine and eleven benzophenanthridine alkaloids often occuring in plants of family papaveraceae.The optimum separation for the benzophenanthridine alkaloids was obtained with Synergi MAX RP 80 A C 12 (4u).

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