Project details


Capillary electrophoresis as a member of the metabolomic analytical toolbox (CE metabolomics)

Project Identification:GAP206/11/0009
Project Period:1/2011 - 12/2015
Investor:link to a new windowCzech Science Foundation
Programme / Project Type:Standard Projects -
MU Faculty/Unit:
Faculty of Science
MU Investigator:Prof. RNDr. Zdeněk Glatz, CSc.
Project Team Member:Mgr. Jindra Smutná, Ph.D.
Other MU Faculty/Unit:
Faculty of Medicine
Project Team Member:Assoc. Prof. MUDr. Igor Crha, CSc.

The generic methods for metabolomic studies based on application of different CE modes - capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MEKC), microemulsion electrokinetic capillary chromatography (MEEKC), nonaqueous capillary electrophoresis (NACE) for corresponding metabolite classes will be developed. The relevant on-line preconcentration strategies will be integrated in given methods and possibility of using with MS detection will be tested. They can be used for metabolite fingerprinting and footprinting, metabolite profiling as well as target analysis. The complete procedure for the target metabolome analysis of microbial samples thus will be available starting from the rapid metabolism quenching and the quantitative extraction of selected metabolites up to their analysis by different CE modes. Besides the new method for the embryo viability testing based on non-invasive metabolite footprinting and profiling will be available as well.