Informace o projektu
Capillary electrophoresis as a member of the metabolomic analytical toolbox
(CE metabolomics)
- Kód projektu
- GAP206/11/0009
- Období řešení
- 1/2011 - 12/2015
- Investor / Programový rámec / typ projektu
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Grantová agentura ČR
- Standardní projekty
- Fakulta / Pracoviště MU
- Přírodovědecká fakulta
- Další fakulta/pracoviště MU
- Lékařská fakulta
The generic methods for metabolomic studies based on application of different CE modes - capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MEKC), microemulsion electrokinetic capillary chromatography (MEEKC), nonaqueous capillary electrophoresis (NACE) for corresponding metabolite classes will be developed. The relevant on-line preconcentration strategies will be integrated in given methods and possibility of using with MS detection will be tested. They can be used for metabolite fingerprinting and footprinting, metabolite profiling as well as target analysis. The complete procedure for the target metabolome analysis of microbial samples thus will be available starting from the rapid metabolism quenching and the quantitative extraction of selected metabolites up to their analysis by different CE modes. Besides the new method for the embryo viability testing based on non-invasive metabolite footprinting and profiling will be available as well.
Publikace
Počet publikací: 46
2016
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Determination of Caffeine and its Metabolites in Saliva and Urine as a Measure of CYP1A2 Metabolic Activity
Current Pharmaceutical Analysis, rok: 2016, ročník: 12, vydání: 4, DOI
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Chapter 11 Bioanalytical methods - Metabolomics
Compendium of Analytical Chemistry - Orange Book, vydání: Vyd. 4, rok: 2016, počet stran: 12 s.
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MEKC LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization
Electrophoresis, rok: 2016, ročník: 37, vydání: 17-18, DOI
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Various instrumental approaches for determination of organic acids in wines
Food Chemistry, rok: 2016, ročník: 194, vydání: March, DOI
2015
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Analysis of amino acids based on capillary electrophoresis with LIF detection using on-capillary derivatization
Rok: 2015, druh: Konferenční abstrakty
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Determination of biochemically important flavins using capillary electrophoresis with laser induced fluorescence detection
Rok: 2015, druh: Konferenční abstrakty
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Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection
Electrophoresis, rok: 2015, ročník: 36, vydání: 11-12, DOI
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Development of capillary electrophoresis based method for analysis of metabolic turnover of energetically important compounds as a tool for human embryo quality assessment
Rok: 2015, druh: Konferenční abstrakty
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Method development for baseline separation of cyanobenz[f]isoindoles of proteinogenic amino acids by CE-LIF
Rok: 2015, druh: Konferenční abstrakty
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Využití kapilární elektroforézy pro stanovení metabolické aktivity embrya
Rok: 2015, druh: Konferenční abstrakty