Detail projektu

 

Capillary electrophoresis as a member of the metabolomic analytical toolbox (CE metabolomics)

Kód projektu:GAP206/11/0009
Období řešení:1/2011 - 12/2015
Investor:odkaz do nového oknaGrantová agentura ČR
Programový rámec / typ projektu:Standardní projekty -
Fakulta/Pracoviště MU:
Přírodovědecká fakulta
Řešitel na MU:prof. RNDr. Zdeněk Glatz, CSc.
Člen realizačního týmu:Mgr. Jindra Smutná, Ph.D.
Další fakulta/pracoviště MU:
Lékařská fakulta
Člen realizačního týmu:doc. MUDr. Igor Crha, CSc.
Publikace/Výsledky:více
Anotace

The generic methods for metabolomic studies based on application of different CE modes - capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MEKC), microemulsion electrokinetic capillary chromatography (MEEKC), nonaqueous capillary electrophoresis (NACE) for corresponding metabolite classes will be developed. The relevant on-line preconcentration strategies will be integrated in given methods and possibility of using with MS detection will be tested. They can be used for metabolite fingerprinting and footprinting, metabolite profiling as well as target analysis. The complete procedure for the target metabolome analysis of microbial samples thus will be available starting from the rapid metabolism quenching and the quantitative extraction of selected metabolites up to their analysis by different CE modes. Besides the new method for the embryo viability testing based on non-invasive metabolite footprinting and profiling will be available as well.