Detail projektu

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Capillary electrophoresis as a member of the metabolomic analytical toolbox (CE metabolomics)

Kód projektu:

GAP206/11/0009

Období řešení:

1/2011 - 12/2015

Investor:

Grantová agentura ČR

Programový rámec / typ projektu:

Standardní projekty -

Fakulta/Pracoviště MU:

Přírodovědecká fakulta
Řešitel na MU:	prof. RNDr. Zdeněk Glatz, CSc.
Člen realizačního týmu:	Mgr. Jindra Musilová

Další fakulta/pracoviště MU:

Lékařská fakulta
Člen realizačního týmu:	doc. MUDr. Igor Crha, CSc.

Publikace/Výsledky:

více

Anotace

The generic methods for metabolomic studies based on application of different CE modes - capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MEKC), microemulsion electrokinetic capillary chromatography (MEEKC), nonaqueous capillary electrophoresis (NACE) for corresponding metabolite classes will be developed. The relevant on-line preconcentration strategies will be integrated in given methods and possibility of using with MS detection will be tested. They can be used for metabolite fingerprinting and footprinting, metabolite profiling as well as target analysis. The complete procedure for the target metabolome analysis of microbial samples thus will be available starting from the rapid metabolism quenching and the quantitative extraction of selected metabolites up to their analysis by different CE modes. Besides the new method for the embryo viability testing based on non-invasive metabolite footprinting and profiling will be available as well.

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