Publication details
Automated spinning disk confocal microscopy in 3D live cell imaging
| Basic information | |
|---|---|
| Original title: | Automated spinning disk confocal microscopy in 3D live cell imaging |
| Authors: | Miroslav Vařecha, Jana Amrichová, Pavel Matula, Michal Kozubek |
| Further information | |
|---|---|
| Citation: | VAŘECHA, Miroslav  - AMRICHOVÁ, Jana - MATULA, Pavel - KOZUBEK, Michal. Automated spinning disk confocal microscopy in 3D live cell imaging. 2009. |
| Original language: | English |
| Field: | Genetics and molecular biology |
| Type: | Vyžádané přednášky |
| Keywords: | fluorescence microscopy; spinning disk; image nalysis |
Fluorescence microscopy has become the leading technology to study structure and dynamics of cellular components and processes. The studies can be performed in two-dimensional (2D) but also in three-dimensional (3D) spatial coordinate system as well as in time and spectral dimensions. Fluorescent proteins allow us to study protein dynamics, localization, and interactions in living cells. In our laboratory, we have been developing special systems for automated cell image acquisition and analysis using fluorescence microscopy working up to five dimensions (x, y, z, t, lambda), whose hardware and software was optimized for studies on living cells. The presentation will focus on the latest developments in our technology. For the first time, we discovered interaction of apoptotic proteins AIF and endonuclease G, expressed using one DNA plasmid, in living human cells during apoptotic cell death.
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