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Image Division Technique in Pre-acquisition Analysis of Information Content for Automated Microscopy

Basic information
Original title:Image Division Technique in Pre-acquisition Analysis of Information Content for Automated Microscopy
Authors:Silvie Luisa Brázdilová, Michal Kozubek
Further information
Citation:BRÁZDILOVÁ, Silvie Luisa - KOZUBEK, Michal. Image Division Technique in Pre-acquisition Analysis of Information Content for Automated Microscopy. Journal of Microscopy, Oxford, Blackwell Science, Great Britain. ISSN 0022-2720, 2011, vol. 242, no. 3, pp. 279-289.
Original language:English
Field:Use of computers, robotics and its application
Type:Article in Periodical
Keywords:Automated microscopy; fluorescence microscopy; autofocusing; multimodal content function; image acquisition; information content analysis

This article presents a method that allows for reliable automated image acquisition of specimens with high information content in light microscopy with emphasis on fluorescence microscopy applications. Automated microscopy typically relies on autofocusing used for the analysis of information content behaviour along z-axis within each field of view. However, in the case of a field of view containing more objects that do not lie precisely in one z-plane, traditional autofocusing methods fail due to their principle of operation. We avoid this issue by reducing the original problem to a set of simple and performable tasks: we divide the field of view into a small number of tiles and process each of them individually. The obtained results enable discovering z-planes with rich information content that remain hidden during global analysis of the whole field of view. Our approach therefore outperforms other acquisition methods including the manual one. A large part of the contribution is oriented toward practical application.

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