Assoc. Prof. Mgr. Lumír Krejčí, Ph.D.

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Person Identification
  • Mgr. Lumir Krejci, Ph.D born 30.3.1972 in Slavicin, married, 3 children
Workplace
  • National Centre for Biomolecular Research
    Masaryk University
    Kamenice 5/A4
    Brno 625 00
    Czech Republic
    Tel: (420) 549493767
    Fax: (420) 549492556
    Email: lkrejci@chemi.muni.cz
    http://ncbr.chemi.muni.cz/lord/
Employment Position
  • Assistant Profesor
Education and Academic Qualifications
  • 1993: B.Sc in Biochemistry, Masaryk University, Brno, Czech Republic
  • 1995: M.Sc in Biochemistry, Masaryk University, Brno, Czech Republic
  • 2000: Ph.D. in Molecular & Cell Biology, Masaryk University, Brno, Czech Republic
Employment Summary
  • 1995-1997: Graduate Fellow, Department of Molecular Biology, Aarhus University, Denmark
  • 1997-2000: Graduate Fellow, Danish Institute of Agricultural Science, Denmark
  • 2000-2001:Research Scientist, Masaryk University, Brno, Czech Republic
  • 2001-2003:Postdoctoral Fellow, Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, TX, USA
  • 2003-2004: Postdoctoral Associate, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA
  • 2004-2005: Associate Research Scientist, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA
  • 2005-present: Associate Professor, National Centre for Biomolecular Research, Masaryk University, Brno, Czech Republic
Pedagogical Activities
  • Study of interactions between proteins and DNA
Scientific and Research Activities
  • 6/1/01-12/1/01: NATO Science Fellowship Programme “Function of human Rad51 paralogs in homologous recombination.”
  • 7/1/03-6/30/06: US DOD Postdoctoral Fellowship BC020457 “Role of Rad51-mediated Interactions in Recombination.”
  • 1/1/06-31/12/11: Wellcome International Senior Research Fellowship "Role of BRCA2 in homologous recombination and genome maintenance".
  • 1/1/06-31/12/09: EMBO-HHMI Young Investigator Programme.
Selected Publications
  • MARINI PALOMEQUE, María Victoria a Lumír KREJČÍ. Unwinding of synthetic replication and recombination substrates by Srs2. DNA Repair, ELSEVIER, 2012, roč. 11, č. 10, s. 789-798. ISSN 1568-7864. doi:10.1016/j.dnarep.2012.05.007. URLinfo
  • ALTMANNOVÁ, Veronika, Peter KOLESÁR a Lumír KREJČÍ. SUMO Wrestles with Recombination. Biomolecules, Basel, Switzerland: MDPI AG, 2012, roč. 2, č. 3, s. 350-375. ISSN 2218-273X. doi:10.3390/biom2030350. Biomoleculesinfo
  • ŠEBESTA, Marek, Peter BURKOVICS, Lajos HARACSKA a Lumír KREJČÍ. Reconstitution of DNA repair synthesis in vitro and the role of polymerase and helicase activities. DNA Repair, 2011, roč. 10, č. 6, s. 567-576. ISSN 1568-7864. doi:10.1016/j.dnarep.2011.03.003. info
  • MARINI PALOMEQUE, María Victoria a Lumír KREJČÍ. Srs2: the Odd-Job Man in DNA repair. DNA Repair, ELSEVIER, 2010, roč. 9, č. 3. ISSN 1568-7864. info
  • MARCO, Saporano, Callahan DEVON, Zheng XIUZHONG, Lumír KREJČÍ, Haber JAMES, Klein HANNAH a Liberi GIORDANO. Cdk1 targets Srs2 to complete synthesis-dependent strand annealing and to promote. PLoS Genetics, Public Library of Science, 2010, roč. 2010, č. 2. ISSN 1553-7390. info
  • ALTMANNOVÁ, Veronika, Nadine ECKERT-BOULET, Milica ARNERIC, Peter KOLESÁR, Radka CHALOUPKOVÁ, Jiří DAMBORSKÝ, Patrick SUNG, Xiaolan ZHAO, Michael LISBY a Lumír KREJČÍ. Rad52 SUMOylation affects the efficiency of the DNA repair. Nucleic Acids Research, Oxford University Press, 2010, roč. 38, č. 6. ISSN 0305-1048. info
  • MATULOVÁ, Petra, María Victoria MARINI PALOMEQUE, RC BURGESS, Alexandra SISÁKOVÁ, Youngho KWON, Rodney ROTHSTEIN, Patrick SUNG a Lumír KREJČÍ. Co-operativity of Mus81-Mms4 with Rad54 in the resolution of recombination and replication intermediates. J Biol Chem., 2009, roč. 284, č. 12, s. 7733-7745. ISSN 1083-351X. info
  • PRAKASH, Rohit, D. SATORY, E. DRAY, A. PAPUSHA, J. SCHELLER, W. KRAMER, Lumír KREJČÍ, Hannah KLEIN, J.E. HABER, Patrick SUNG a Grzegorz IRA. Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Genes Dev., 2009, roč. 23, č. 1, s. 67-79. ISSN 1549-5477. info
  • ANTONY, Edwin, Eric J. TOMKO, Qi XIAO, Lumír KREJČÍ, Timothy M. LOHMAN a Tom ELLENBERGER. Srs2 Disassembles Rad51 Filaments by a Protein-Protein Interaction Triggering ATP Turnover and Dissociation of Rad51 from DNA. Molecular Cell, 2009, roč. 35, č. 1, s. 105-115. ISSN 1097-2765. info
  • BURGESS, R.C., Michael LISBY, Veronika ALTMANNOVÁ, Lumír KREJČÍ, Patrick SUNG a Rodney ROTHSTEIN. Localization of recombination proteins and Srs2 reveals anti-recombinase function in vivo. Journal of Cell Biology, 2009, roč. 185, č. 6, s. 969-981. ISSN 0021-9525. info
  • SEONG, Changhyuan, Siera COLAVITO, YongHo KWON, Patrick SUNG a Lumír KREJČÍ. Regulation of Rad51 recombinase presynaptic filament assembly via interactions with the Rad52 mediator and the Srs2 anti-recombinase. J Biol Chem., 2009, roč. 254, č. 12, s. 104-111. ISSN 0021-9258. info
  • SIERRA, Colavito, Macris-Kiss MARGARET, Seong CHANGHYUN, Gleeson OLIVE, Greene ERIC C., Klein HANNAH L., Krejci LUMIR a Sung PATRICK. Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption. Nucleic Acids Research, ENGLAND: OXFORD UNIV PRESS, 2009, roč. 20, č. 37, s. 6754-64. ISSN 0305-1048. URLinfo
  • SEONG, Changhyuan, Michael SEHORN, Iben PLATE, Idina SHI, Binway SONG, Peter CHI, Uffe MORTENSEN, Patrick SUNG a Lumír KREJČÍ. Molecular anatomy of the recombination mediator function of Saccharomyces cerevisiae Rad52. J. Biol. Chem., 2008, roč. 18, č. 283, s. 12166-74. ISSN 0021-9258. URLinfo
  • KREJČÍ, Lumír. UPS AND DOWNS OF HOMOLOGOUS RECOMBINATION. 2007. URLinfo
  • KREJČÍ, Lumír. DNA Binding and Strand Annealing Properties of the Human BRCA2 DNA Binding Domain. Paris: Institute Curie, 2006. URLinfo
  • MACRIS, Margaret, Lumír KREJČÍ, Wendy BUSSEN, Akira SHIMAMOTO a Patrick SUNG. Biochemical characterization of the RECQ4 protein, mutated in Rothmund-Thomson syndrome. DNA Repair, ELSEVIER, 2006, roč. 2, č. 5, s. 172-180. ISSN 1568-7864. URLinfo
  • FILLIPO, Joseph, Peter CHI, Lumír KREJČÍ a Patrick SUNG. Recombination Mediator and Rad51 Targeting Activities of a Human BRCA2 Polypeptide. J. Biol. Chem., 2006, roč. 17, č. 281, s. 11649-11657. ISSN 0021-9258. URLinfo
  • KREJČÍ, Lumír. Srs2 protein and its multi-functional role in recombination/repair processes. Hiedelberg: EMBO, 2006. URLinfo
  • KREJČÍ, Lumír. Srs2 protein and its multi-functional role in recombination/repair processes. Dubrovník: HHMI, 2006. URLinfo
  • MACRIS, MA, Lumír KREJČÍ, W. BUSSEN, A. SHIMAMOTO a Patrick SUNG. Biochemical characterization of the RECQ4 protein, mutated in Rothmund-Thomson syndrome. DNA Repair, 2006, roč. 5, č. 2. ISSN 1568-7864. info
  • KREJCI, Lumir, Iben PLATE, Patrick SUNG, Michael SEHORN, Wendy BUSSEN a Uffe H. MORTENSEN. New insight into mediator function of yeast Rad52 protein. In XXIIth International Conference on Yeast Genetics and Molecular Biology. Bratislava, Slovakia: ASM Conferences Program, 2005. 90 s. URLinfo
  • KREJCI, Lumir, Julia ETCHIN, San Joe FILIPPO a Patrick SUNG. DNA Binding and Strand Annealing Properties of the Human BRCA2 DNA Binding Domain. In Era of Hope 2005 - Department of Defense Breast Cancer Research Program Meeting. Philadelphia, USA: U.S. Army Medical Research and Materiel Command, 2005. 89 s. URLinfo
  • CHEN, ling, Kelly TRUJILLO, Stephen VAN KOMEN, Dong ROH, Lumír KREJČÍ, Kevin LEWIS, Mike RESNICK, Patrick SUNG a Alan TOMKINSON. Effect of amino acid substitutions in the rad50 ATP binding domain on DNA double strand break repair in yeast. Journal of Biological Chemistry, Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2005, roč. 280, č. 4, s. 2620-7. ISSN 0021-9258. URLinfo
  • PRAKASH, Rohit, Lumír KREJČÍ, Stephen VAN KOMEN, Anke SCHURER, Wilfried KRAMER a Patrick SUNG. Saccharomyces cerevisiae MPH1 gene, required for homologous recombination-mediated mutation avoidance, encodes a 3' to 5' DNA helicase. Journal of Biological Chemistry, Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2005, roč. 280, č. 9, s. 7854-60. ISSN 0021-9258. URLinfo
  • PAPOULI, Efterpi, Shuhua CHEN, Adelina DAVIES, Diana HUTTNER, Lumir KREJCI, Patrick SUNG a Helle ULRICH. Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2p. Molecular Cell, 2005, roč. 19, č. 1, s. 123-33. ISSN 1097-2765. URLinfo
  • KREJČÍ, Lumír, Margaret MACRIS, Stephen VAN KOMEN, Jane VILLEMAIN, Tom ELLENBERGER, Hannah KLEIN a Patrick SUNG. Role of ATP hydrolysis in the antirecombinase function of Saccharomyces cerevisiae Srs2 protein. Journal of Biological Chemistry, Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2004, roč. 279, č. 22, s. 23193-9. ISSN 0021-9258. URLinfo
  • KREJČÍ, Lumír, Stephen VAN KOMEN, Ying LI, Jane VILLEMAIN, Sredhar REDDY, Hannah KLEIN, Tom ELLENBERGER a Patrick SUNG. DNA helicase Srs2 disrupts the Rad51 presynaptic filament. Nature, Lodon, UK: Nature Publishing Group, 2003, roč. 423, č. 6937, s. 305-9. ISSN 0028-0836. info
  • SUNG, Patrick, Lumír KREJČÍ, Stephen VAN KOMEN a Michael SEHORN. Rad51 recombinase and recombination mediators. Journal of Biological Chemistry, Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2003, roč. 278, č. 44, s. 42729-32. ISSN 0021-9258. info
  • VAN KOMEN, Stephen, Sredhar REDDY, Lumír KREJČÍ, Hannah KLEIN a Patrick SUNG. ATPase and DNA helicase activities of the Saccharomyces cerevisiae anti-recombinase Srs2. Journal of Biological Chemistry, Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2003, roč. 278, č. 45, s. 44331-7. ISSN 0021-9258. info
  • KREJČÍ, Lumír, Ling CHEN, Stephen VAN KOMEN, Patrick SUNG a Alan TOMKINSON. Mending the break: two DNA double-strand break repair machines in eukaryotes. Prog Nucleic Acid Res Mol Biol., 2003, roč. 2003, č. 74, s. 159-201. URLinfo
  • MADSEN, L, A PETERSEN, V NIELSEN, P NISSEN, Morten DUNO, Lumír KREJČÍ, Christian BENDIXEN a Bo THOMSEN. Chromosome location, genomic organization of the porcine COL10A1 gene and model structure of the NC1 domain. Cytogenet Genome Res., Basel, 2003, roč. 102, 1-4, s. 173-8. ISSN 1424-8581. URLinfo
  • KREJCI, Lumir a Patrick SUNG. RPA not that different from SSB. Structure, USA: CELL PRESS, 1100 MASSACHUSETTS AVE, CAMB, 2002, roč. 10, č. 5, s. 601-2. ISSN 0969-2126. info
  • KREJCI, Lumir a Patrick SUNG. Of forks and ends. Trends in biochemical sciences, Cambridge: Elsevier Science, 2002, roč. 27, č. 5, s. 225-6. ISSN 0968-0004. info
  • KREJCI, Lumir, Binwei SONG, Wendy BUSSEN, Rodney ROTHSTEIN, Uffe MORTENSEN a Patrick SUNG. Interaction with Rad51 is indispensable for recombination mediator function of Rad52. Journal of Biological Chemistry, Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2002, roč. 277, č. 42, s. 40132-41. ISSN 0021-9258. info
  • DUNO, Morten, Bo THOMSEN, Ole WESTERGAARD, Lumír KREJČÍ a Christian BENDIXEN. Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1. Mol Gen Genet, 2000, roč. 264, 1-2, s. 89-97. ISSN 1617-4615. info

Last update: 2007/01/11