Project details


Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

Project Identification:LC06035
Project Period:3/2006 - 12/2011
Investor:link to a new windowMinistry of Education, Youth and Sports of the CR
MU Faculty/Unit:
Faculty of Science
MU Investigator:Assoc. Prof. RNDr. Libuše Trnková, CSc.
Cooperating Organization:
link to a new windowInstitute of Biophysics
link to a new windowFaculty of Chemistry and Technology Univ. Pardubice
link to a new windowCharles University Prague
link to a new windowMasaryk Memorial Cancer Institute Brno
link to a new windowNatural Science Faculty CU Praha
link to a new windowUniversity of Pardubice
Field:CE - Biochemistry (C - Chemistry)
CG - Electrochemistry (C - Chemistry)
EB - Genetics and molecular biology (E - Bio-science)
Keywords:nukleové kyseliny, proteiny, karcinogeny, léčiva, poškození DNA, hybridizace DNA, interakce DNA-protein, nádorové supresory, amyloidní proteiny, mutantní proteiny, denaturace, agregace, elektrochemie, separace, kapilární elektroforéza, hmotová spektromet

After completion of the human genome project, there is demand for simple and inexpensive methods enabling analysis of details of individuals' genomes. The contemporary world science is oriented towards utilization of electrochemical DNA detection in thisanalysis. DNA electroactivity was discovered at the Institute of Biophysics in Brno. Laboratories of this Institute belong to the world elite in this field for more than four decades. In the partner laboratories, a considerable progress has been attainedin the research of electrochemical DNA sensors as well as in analysis of proteins and other substances in recent years. This project is based on the existing results and it is oriented towards advanced studies of the properties of biomacromolecules andt heir interactions with surfaces, especially electrodes, and towards research of intermolecular interactions of DNA and/or proteins important in severe disorders (cancer, Parkinson's or Alzheimer's disease). On the basis of electrochemistry, miniaturiza