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Use of the manganese-dependent superoxide dismutase gene sodA for rapid identification of recently described enterococcal species

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FROLKOVÁ Petra GHOSH Anuradha ŠVEC Pavel ZUREK Ludek LITERÁK Ivan

Rok publikování 2012
Druh Článek v odborném periodiku
Časopis / Zdroj Folia Microbiologica
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.1007/s12223-012-0115-8
Obor Mikrobiologie, virologie
Klíčová slova manganese-dependent superoxide dismutase; sodA; Enterococcus; identification; taxonomy
Popis Enterococci are a clinically important group of bacteria for humans and animals. Over the past decade, the genus Enterococcus has been studied using various classification methods and the number of newly described species increased rapidly. Poyart et al. used amplification and sequencing of the internal DNA fragment of the manganese-dependent superoxide dismutase gene sodA for identification of Gram-positive cocci. The degenerate primers were designed for identification of streptococci, and lately, a database of specific sequences of 19 enterococcal species was constructed. In the current study, we aimed to evaluate this method to identify another 18 recently described enterococcal species.
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