Lysosomal pathway of the TRAIL-induced apoptosis in breast carcinoma cells-the role of the lysosomal protease cathepsin D

• Autoři: JANČEKOVÁ Blanka; ONDROUŠKOVÁ Eva; KNOPFOVÁ Lucia; BENEŠ Petr; ŠMARDA Jan
• Rok publikování: 2013
• Druh: Další prezentace na konferencích
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• Popis: In this study, we investigated the role of lysosomal aspartyl protease cathepsin D (CTSD( in the TRAIL-induced apoptosis. We showed that overexpression of CTSD sensitizes MDA-MB-231 breast carcinoma cells to the TRAIL-induced apoptosis in enzymatic activity-dependent manner. The increased frequency fo apoptosis observed in the CTSD-overexpressing cells corresponded to caspase activation and cleavage of the Bid protein, the pro-apoptotic BCL-2 family member. We also showed that the CTSD-mediated pro-apoptotic effect can be partially reversed by the caspase 8 and aspartyl protease inhibitors.

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