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Expression, function and regulation of Evi-1 during embryonic avian development

Název česky Exprese, funkce a regulace genu Evi-1 během embryonáního vývoje ptáků.
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CELÁ Petra MORAVCOVÁ BALKOVÁ Simona BRYJOVÁ Anna HORÁKOVÁ Dana MÍŠEK Ivan RICHMAN J M BUCHTOVÁ Marcela

Rok publikování 2013
Druh Článek v odborném periodiku
Časopis / Zdroj Gene Expression Patterns
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www http://www.sciencedirect.com/science/article/pii/S1567133X13000720#
Doi http://dx.doi.org/10.1016/j.gep.2013.06.002
Obor Ostatní lékařské obory
Klíčová slova Ecotropical viral integration site 1; Chondrogenesis; siRNA; Limb patterning; Anterior-posterior axes; Pharyngeal arch; Craniofacial
Přiložené soubory
Popis Ecotropical viral integration site 1 (Evi-1) is a transcription factor essential for vascularisation and cell proliferation during embryonic development. The chimeric transcription factor AML1-EVI-1 is activated in leukaemia where it plays a role as a differentiation block and stimulator of proliferation. Here, we cloned chicken Evi-1 and analysed its expression during embryonic development. There was early expression in the pharyngeal arches, in the brain and intermediate mesoderm of chicken embryos at stage 15. Later at stage 20, Evi-1 mesenchymal expression was concentrated in the second pharyngeal arch, and weaker expression was found in the mandibular and maxillary prominences. Facial expression decreased in intensity during development. Evi-1 expression in the limb was also limited to the mesenchyme with the most prominent expression in the anterior margin. Evi-1 was not detectable in the posterior limb bud. At later stages, Evi-1 was expressed in the peripheral mesenchyme of the limb but not in the developing precartilage blastema. At stage 29, the expression became restricted to the perichondrium and interdigital areas; however, the cartilage condensations themselves were negative. To study the function of Evi-1 in chondrogenesis, we knocked down expression in limb micromass cultures using siRNA. Chondrogenesis was significantly reduced in both anterior and posterior cultures. Since Evi-1 was expressed adjacent to the apical ectodermal ridge and this area is a source of FGFs, we tested whether endogenous FGF receptor signalling was necessary to maintain its expression. Inhibitors of FGFRs (PD161570 and SU5402) were applied to wing mesenchyme, and downregulation of Evi-1 expression was observed after treatment with both inhibitors. Therefore, Evi-1 may be a transcription factor mediating the effects of FGF and may also be defining the size of cartilage elements in the limb.
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