Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

• Autoři: VESELSKÁ Renata; NERADIL Jakub; NEKULOVÁ Marta; DOBRUCKÁ Lucia; VOJTĚŠEK Bořivoj; ŠTĚRBA Jaroslav; ZITTERBART Karel
• Rok publikování: 2013
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Histology and Histopathology
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• www: http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm
• Obor: Genetika a molekulární biologie
• Klíčová slova: medulloblastoma;deltaNp73;immunocytochemistry;immunoblotting;transmission electron microscopy
• Popis: This study is focused on the intracellular localization of Delta Np73 in medulloblastoma cell lines. Due to a lack of suitable anti-Delta Np73 antibodies, we developed two new rabbit polyclonal antibodies, Delta Np7326 and Delta Np73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the Delta Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the Delta Np73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the Delta Np73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with Delta Np73 and golgin-97, we showed the co-localization of the Delta Np73 isoform with the Golgi apparatus.

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