Informace o publikaci
Piezoelectric biosensors for detection of microorganisms
| Autoři | |
|---|---|
| Rok publikování | 2014 |
| Druh | Článek ve sborníku |
| Konference | XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://web2.mendelu.cz/af_239_nanotech/data/soubory/sbornik2014.pdf#page=34 |
| Obor | Biochemie |
| Klíčová slova | Biosensor; Escherichia coli; Immunosensor; QCM |
| Popis | Piezoelectric biosensors provide fast, specific and economic way of detection for a wide range of analytes ranging from metabolites to microorganisms. Specific antibodies were immobilised on gold electrodes of piezoelectric crystal and the biosensor was applied for real-time detection of model microorganisms. Three nonpathogenic strains of Escherichia coli (BL21, DH5 alpha and K-12) were used because of easy cultivation and availability of antibodies. Several methods of antibody immobilisation were compared. The immobilisation of reduced antibody using Sulfo-SMCC was the most effective achieving the limit of detection (LOD) 10^5 CFU·mL-1. Active and passive operation modes of quartz crystal microbalance were compared concluding that for detection of microorganisms, both approaches are practically identical. The developed biosensor was connected to the cyclone air sampler allowing detection of microorganisms disseminated in the form of aerosol inside bioaerosol chamber. The achieved LOD was 1.45·10^4 CFU·L-1 of air and the time from sample collection to detection was 16 min. |
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