Informace o publikaci

All-in-one detector of circulating mRNA based on a smartphone

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CMIEL Vratislav GUMULEC Jaromír SVOBODA Ondrej RAUDENSKÁ Martina HUDCOVÁ Kristýna SEKORA Jiri BALOGH Jaroslav MASAŘÍK Michal PROVAZNIK Ivo

Rok publikování 2016
Druh Článek ve sborníku
Konference Proceedings of SPIE : Optical Diagnostics and Sensing XVI - Toward Point-of-Care Diagnostics
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1117/12.2213253
Obor Ostatní obory vnitřního lékařství
Klíčová slova mRNA; smartphone; PCR; cancer; metallothionein
Popis Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.

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