Informace o publikaci

Site-Directed Conjugation of Antibodies to Apoferritin Nanocarrier for Targeted Drug Delivery to Prostate Cancer Cells

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DOSTALOVA Simona CERNA Tereza HYNEK David KOUDELKOVA Zuzana VACULOVIČ Tomáš KOPEL Pavel HRABETA Jan HEGER Zbyněk VACULOVICOVA Markéta ECKSCHLAGER Tomáš STIBOROVA Marie ADAM Vojtěch

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj ACS APPLIED MATERIALS & INTERFACES
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.1021/acsami.6b04286
Obor Analytická chemie, separace
Klíčová slova antibodies; apoferritin; doxorubicin; nanomedicine; targeted drug delivery
Popis Herein, we describe a novel approach for targeting of ubiquitous protein apoferritin (APO)-encapsulating doxorubicin (DOX) to prostate cancer using antibodies against prostate specific membrane antigen (PSMA). The conjugation of anti-PSMA antibodies and APO was carried out using HWRGWVC heptapeptide, providing their site-directed orientation. The prostate cancer-targeted and nontargeted nanocarriers were tested using LNCaP and HUVEC cell lines. A total of 90% of LNCaP cells died after treatment with DOX (0.25 mu M) or DOX in nontargeted and prostate-cancer-targeted APO, proving that the encapsulated DOX toxicity for LNCaP cells remained the same. Free DOX showed higher toxicity for nonmalignant cells, whereas the toxicity was lower after treatment with the same dosage of APO-encapsulated DOX (APODOX) and even more in prostate-cancer-targeted APODOX. Hemolytic assay revealed exceptional hemocompatibility of the entire nanocarrier. The APO encapsulation mechanism ensures applicability using a wide variety of chemotherapeutic drugs, and the presented surface modification enables targeting to various tumors.
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