Informace o publikaci

Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes

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BUCHTOVÁ Žaneta LACKOVÁ Zuzana KUDR Jiří ŠKODA Jan ZÍTKA Ondřej ZÍTKA Zdeněk

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj Molecules
Fakulta / Pracoviště MU

Fakulta sportovních studií

Citace
www https://www.mdpi.com/1420-3049/23/10/2504
Doi http://dx.doi.org/10.3390/molecules23102504
Klíčová slova antioxidant molecules; blood drop analysis; electrochemical analysis; nutritional study; sample pretreatment
Popis Glutathione ( -glutamyl-cysteinyl-glycine; also known as GSH) is an endogenous antioxidant that plays a crucial role in cell defense mechanisms against oxidative stress. It is thus not surprising that this molecule can serve as a biomarker for oxidative stress monitoring. As capillary blood is a highly accessible target for biomarking, it is a valuable bodily fluid for diagnosing human GSH levels. This study focused on the optimization of GSH measurements from micro volumes of capillary blood prior to using electrochemical detection. The optimization of experimental parameters, including the sample volume and its stability, was performed and evaluated. Moreover, we tested the optimized method as part of a short-term study. The study consisted of examining 10 subjects within 96 h of their consumption of high amounts of antioxidants, attained from a daily dose of 2 g/150 mL of green tea. The subjects’ capillary blood (5 L) was taken at 0 h, 48 h, and 96 h for subsequent analysis. The short-term supplementation of diet with green tea
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