Informace o publikaci

Role of Vertical Transmission of Shoot Endophytes in Root-Associated Microbiome Assembly and Heavy Metal Hyperaccumulation in Sedum alfredii

Autoři	LUO Jipeng TAO Qi JUPA Radek LIU Yuankun WU Keren SONG Yuchao LI Jinxing HUANG Yue ZOU Linyun LIANG Yongchao LI Tingqiang
Rok publikování	2019
Druh	Článek v odborném periodiku
Časopis / Zdroj	ENVIRONMENTAL SCIENCE & TECHNOLOGY
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
www	https://pubs.acs.org/doi/10.1021/acs.est.9b01093
Doi	http://dx.doi.org/10.1021/acs.est.9b01093
Klíčová slova	Comamonadaceae; contaminated soils; root microbiota; phytoremediation; soil irradiation; Streptomycetaceae
Popis	The transmission mode of shoot-associated endophytes in hyperaccumulators and their roles in root microbiome assembly and heavy metal accumulation remain unclear. Using 16S rRNA gene profiling, we investigated the vertical transmission of shoot-associated endophytes in relation to growth and Cd/Zn accumulation of Sedum alfredii (Crassulaceae). Endophytes were transmitted from shoot cuttings to the rhizocompartment of new plants in both sterilized (gamma-irradiated) and native soils. Vertical transmission was far more efficient in the sterile soil, and the transmitted endophytes have become a dominant component of the newly established root-associated microbiome. Based on 16S rRNA genes, the vertically transmitted taxa were identified as the families of Streptomycetaceae, Nocardioidaceae, Pseudonocardiaceae, and Rhizobiaceae. Abundances of Streptomycetaceae, Nocardioidaceae, and Pseudonocardiaceae were strongly correlated with increased shoot biomass and total Cd/Zn accumulation. Inoculation of S. alfredii with the synthetic bacterial community sharing the same phylogenetic relatedness with the vertically transmitted endophytes resulted in significant improvements in plant biomass, root morphology, and Cd/Zn accumulation. Our results demonstrate that successful vertical transmission of endophytes from shoots of S. alfredii to its rhizocompartments is possible, particularly in soils with attenuated microbiomes. Furthermore, the endophyte-derived microbiome plays an important role in metal hyperaccumulation.