Informace o publikaci
Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR-RFLP of kdr mutation in sodium channel gene
| Autoři | |
|---|---|
| Rok publikování | 2019 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Experimental and Applied Acarology |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://link.springer.com/article/10.1007%2Fs10493-019-00353-9 |
| Doi | http://dx.doi.org/10.1007/s10493-019-00353-9 |
| Klíčová slova | Varroa destructor; Honey bee; Apiary; Acaricide; Restriction |
| Popis | In this study, we used the discriminating concentration of tau-fluvalinate (0.25 mu g/mL) to detect the resistance of mites in a bioassay. Further, we verified the presence of the kdr mutation in mites from the bioassay via PCR amplification of a fragment of the voltage-gated sodium channel gene (Na(V)Ch), restriction fragment length polymorphisms (RFLPs), and densitometry analyses in pools of surviving or dead mites. |