Informace o publikaci

Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma

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BOHMOVA Jana LUBUSKY Marek HOLUSKOVA Iva STUDNICKOVA Martina KRATOCHVILOVA Romana KREJCIRIKOVA Eva DURDOVA Veronika KRATOCHVILOVA Tereza DUŠEK Ladislav PROCHAZKA Martin VODICKA Radek

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj Diagnostics
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.mdpi.com/2075-4418/10/8/564
Doi http://dx.doi.org/10.3390/diagnostics10080564
Klíčová slova non-invasive fetal genotyping; RHDgene; cell-free fetal DNA; real-time PCR; QF PCR; Rh blood group system; red blood cell alloimmunization; hemolytic disease of the fetus and newborn
Popis Noninvasive fetalRHDgenotyping is an important tool for predicting RhD incompatibility between a pregnant woman and a fetus. This study aimed to assess a methodological approach other than the commonly used one for noninvasive fetalRHDgenotyping on a representative set of RhD-negative pregnant women. The methodology must be accurate, reliable, and broadly available for implementation into routine clinical practice. A total of 337 RhD-negative pregnant women from the Czech Republic region were tested in this study. The fetalRHDgenotype was assessed using two methods: real-time PCR and endpoint quantitative fluorescent (QF) PCR. We used exon-7-specific primers from theRHDgene, along with internal controls. Plasma samples were analyzed and measured in four/two parallel reactions to determine the accuracy of theRHDgenotyping. TheRHDgenotype was verified using DNA analysis from a newborn buccal swab. Both methods showed an excellent ability to predict theRHDgenotype. Real-time PCR achieved its greatest accuracy of 98.6% (97.1% sensitivity and 100% specificity (95% CI)) if all four PCRs were positive/negative. The QF PCR method also achieved its greatest accuracy of 99.4% (100% sensitivity and 98.6% specificity (95% CI)) if all the measurements were positive/negative. Both real-time PCR and QF PCR were reliable methods for precisely assessing the fetalRHDallele from the plasma of RhD-negative pregnant women.

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