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Establishment of human embryonic stem cells for stem-cell therapies

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SOURALOVÁ Tereza VENTRUBA Pavel JEŠETA Michal ŘEHÁKOVÁ Daniela HAMPL Aleš KOUTNÁ Irena

Rok publikování 2022
Druh Konferenční abstrakty
Citace
Popis Human embryonic stem cells are able to differentiate into every cell type in the human body making them a tremendous cell source for regenerative medicine. However, the production of human embryonic stem cell lines for clinical use is challenging as clean rooms, highly-qualified personnel, standard operating procedures for both manufacture and quality control are required. Here we present the derivation of clinical-grade hESC lines in cooperation with the Centre of assisted reproduction - University Hospital Brno which provided 6-day old blastocysts. Laminin 521 in combination with Nutristem, Human serum albumin, and ECadherin was used for the mechanical derivation. Human embryonic stem cells were cultured on laminin 521 in a Nutristem medium. The derivation itself and subsequent culture are xeno-free, feeder-free, and fulfill standards of current good manufacturing practices. In-depth quality control provides essential information about the safety, pluripotency, and differentiation potential of lines. Presented clinical-grade hESC lines were established according to state-of-the-art technology that makes them excellent cell source for stem cell-based therapies.
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