Informace o publikaci

Comparison of the bioluminescence of Photorhabdus species and subspecies type strains

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HYRŠL Pavel ČÍŽ Milan LOJEK Antonín

Rok publikování 2004
Druh Článek v odborném periodiku
Časopis / Zdroj Folia Microbiologica
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Popis Natural bioluminescence of genus Photorhabdus, bacterial symbionts of entomopathogenic nematodes was studied. Bioluminescence of all recently accepted Photorhabdus species and subspecies type strains was measured using a commercial luminometer. Optimal conditions for the measurement and comparison of all species and subspecies are described. Cultures of all tested strains emitted reliably measurable bioluminescence, with characteristic level and kinetics for each strain. Bioluminescence of all strains was significantly higher at 37 C when compared with 25 C at the beginning of the measurement, no effect of bacterial concentration on the intensity of bioluminescence was observed in our experimental conditions. The technique described can provide reliable and quick information for determination of Photorhabdus taxons.

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