Molecular and Cell Biology and Genetics

Doctoral degree in full-time or combined form. The language of instruction is Czech.

The programme can be studied only as a single subject.

Application deadline depending on the admission procedure chosen (midnight 30 April 2024 or 15 December 2024)

What will you learn?

The program is product of fusion of former independent Molecular and Cellular Biology with General and Molecular Genetics. We propose the fusion to reflect modern holistic approaches dominating in both fields that converge them to close proximity and bringing benefits to both of them. The aim of the program is providing excellent scientific education in the field of molecular and cell biology and genetics. The graduates thus should be proficient to accomplish research of living phenomena on molecular, cellular, tissue or organismal levels. To achieve this aim, students are systematically guided to advance their theoretical knowledge in the field and master practical skills in applications of modern methods of molecular and cellular biology, genetics and other related fields. The key themes include study of genes and genomes and their expression in microorganisms, plants, animals and humans. Special attention is paid to their relations to pathological conditions. Research performed on microorganisms is focused preferentially on molecular diagnosis and genomics of selected pathogenic and clinically significant bacterial strains and their interactions with bacteriophages. Research of plants is concentrated mainly on genes of model plants and plants used in agriculture. In animals and humans, research is focused on genetical structure of populations, molecular diagnosis of prenatal and postnatal pathogenic situations, genetics of tumors, study of signalling processes connected with deregulation of proliferation, differentiation and programmed cell death in tumor cells and detection of genetical factors associated with certain polygenic diseases. Students are free to perform independent research in well-equipped laboratories and experienced supervisors are nominated to guide them in this effort. Students are continuously confronted with progress in the field by discussions in regular laboratory meetings, institutional seminars or conferences. Students successfully presenting their results in conferences or written articles can be awarded by special scholarships for excellent representation of the Deparmtent of Experimental Biology and following activities:

1. Successfull presentation record resulting from reaching aims of Ph.D. thesis projects, such as:

  • papers in international journals or chapters in monographies, considering ranking of the journal (IF, Q), placings of the student among co-authors, number of co-authors, proportion of the student efforts on the published results.
  • presentation of the results on conferencies, considering placings of the student amnog co-authors, number of co-authors, proportion of the student efforts on the published results and the type of the conference (international or domestic).

The scholarship can be used for partial covering of travel/accomodation expenses or conference fees.

The essential condition for awarding the scholarship is correct affiliation of the presentations (both papers and conference abstracts) to the Deparment of Experimental Biology, Faculty of Science, Masaryk University.

2. Participation in teaching younger students of Master programmes how to perform experimental methods and interprete the results obtained.

3. Participation in grant projects. Long-lasting participation and significant contribution to accomplishing the projects aims can be awarded by getting part-time job or contract for certain period.

“Windows of the living cell universe wide-opened.”

Practical training

The students who are interested in applied research can collaborate with companies as Repromeda (assisted reproduction), MB Pharma (devising phage preparations) or to participate in grant projects funded by TACR leading to applied outcomes. We aim to further support contractual research with applied potential, search for suitable partners and provide them with option to collaborate with students interested in this kind of research.

Further information

Additional information can be found in following addresses:

http://www.sci.muni.cz/cz/DoktorskeStudium/Prehled-programu-a-oboru

http://www.sci.muni.cz/cz/UEB

The Office for Doctoral Studies, Quality, Academic Affairs and Internationalization takes care of doctoral students SCI MU

https://www.sci.muni.cz/en/students/phd

On the department's website, you can find the following information:

  • Forms (application forms for state examinations and defences, various applications, etc. )
  • Legislation (links to: MU Study and Examination Regulations, Scholarship Regulations of MU, Terms of Scholarship Programmes of the Faculty of Science)
  • Dissertations (Guidelines for dissertations, templates)
  • Manuals (guidelines for Individual Study Plans, study and research obligations in DSP, etc.)
  • Doctoral study programmes (recommended study plans, examination committees, overview of accredited programmes)
  • Deadlines for the doctoral state examinations and defences
  • Enrolment (information needed for the enrolment to the next semester)
  • Graduation

but also office hours, contacts, news, information on skills development and scholarships.

Detailed information on stays abroad can be found on this website:

https://www.sci.muni.cz/en/students/phd/develop-your-skills/stay-abroad

Career opportunities

Graduates find positions in various research institutes, universities, hospitals and other medical facilities and laboratories oriented to virology, microbiology, genetics, biochemistry, immunology, pharmacology, pathology, etc. They are ready to perform independent research, draft scientific projects, create grant applications, design experimental work itself, interprete results rigorously and present them in oral as well as written forms. They are also educated to act as teachers. Graduates from this program are sought-after by employers and many of them currently work on positions of leading researchers, university teachers, top managers and directors in various research and education institutions in Brno, Prague, Ostrava, České Budějovice, Olomouc, etc. Many graduates leaves for postdoctoral stays abroad, especially to west-european countries, USA, Canada, Japan, Australia. They often become highly-appreciated members of research teams there.

Admission requirements

Admission to Doctoral degree programmes in 2024/2025 (beginning: Autumn 2024)
— Submission deadline until midnight 30 Apr 2024

Admission procedure
The admission interview is usually in an online form and consists of two parts:
1) Expert interview – checking the knowledge of molecular and cell biology and genetics at the master's level, emphasising the ability to put the findings into a general context. The examination focuses mainly on the planned topic of the PhD thesis (max. 100 points),
2) Language part – checking the applicants' ability to present their current scientific (diploma) work and the concept of their planned PhD research in English. (max. 100 points)

More information about admission process for international applicants in general can be found here.

Date of the entrance exam
The applicants will receive information about the entrance exam by e-mail usually at least 10 days before the exam.
Please, always check your e-mails, including spam folders.

Conditions of admission
To be admitted, a candidate must obtain a total of 70 out of 100 points in the expert knowledge part and 60 out of 100 points in the language part.
Successful applicants are informed of their acceptance by e-mail and subsequently receive an invitation to the enrolment.

Programme capacity
The capacity of a given programme is not fixed; students are admitted based on a decision by the Doctoral Board after assessing their aptitude for study and motivation.

International applicants for doctoral study (Czech and Slovak Republics applicants NOT included)
— Submission deadline until midnight 15 Dec 2024

Admission procedure
The admission interview is usually in an online form and consists of two parts:
1) Expert interview – checking the knowledge of molecular and cell biology and genetics at the master's level, emphasising the ability to put the findings into a general context. The examination focuses mainly on the planned topic of the PhD thesis (max. 100 points),
2) Language part – checking the applicants' ability to present their current scientific (diploma) work and the concept of their planned PhD research in English. (max. 100 points)

More information about admission process for international applicants in general can be found in the section Admission Process.

Date of the entrance exam
The applicants will receive information about the entrance exam by e-mail usually at least 10 days before the exam.
Please, always check your e-mails, including spam folders.

Conditions of admission
To be admitted, a candidate must obtain a total of 70 out of 100 points in the expert knowledge part and 60 out of 100 points in the language part.
Successful applicants are informed of their acceptance by e-mail and subsequently receive an invitation to the enrolment.

Programme capacity
The capacity of a given programme is not fixed; students are admitted based on a decision by the Doctoral Board after assessing their aptitude for study and motivation.

Deadlines

1 Jan – 30 Apr 2024

Submit your application during this period

Admission to Doctoral degree programmes in 2024/2025 (beginning: Autumn 2024)

2 Jan – 15 Dec 2024

Submit your application during this period

International applicants for doctoral study (Czech and Slovak Republics applicants NOT included)

Dissertation topics

Single-subject studies

Molecular pathology and personalized approach in oncology
Supervisor: RNDr. Ing. Ondřej Bonczek, Ph.D.

Řada genových mutací, které způsobují vývojové abnormality, např. orofaciální vady, jsou sdílené s onkologickými onemocněními a jsou zodpovědné rovněž za indukci karcinogeneze. Nicméně současné znalosti o asociacích sdílených molekulárních charakteristik mezi orofaciálními anomáliemi a karcinogenezí jsou minimální. Cílem práce bude zaměřit se na současný výskyt orofaciálních abnormalit a nádorových onemocnění. Dále zjistit, zda by bylo možné využít znalosti sdílené asociace genů a nalezených genetických variant např. k cílenému časnému záchytu rakoviny. Metodicky se bude dizertační práce zabývat především přípravou exomových a multigenových sekvenačních knihoven, eventuálně dalšími metodami analýzy genetických změn. Mimo včasného screeningu rakoviny se bude dizertační práce zabývat zajímavými genomovými změnami, které jsou detekované při molekulárním profilování nádorů v rámci rutinních prediktivních vyšetření. Jedná se např. o sekundární reverzní mutace, varianty identifikované v Kozakově sekvenci, varianty v oblastech sestřihu exonů apod. Obvykle však nejsou tyto aberace hlouběji studovány a popisovány, protože je to již nad rámec aktuálního klinického využití. Nalezené genetické změny budou detailně popsány z hlediska jejich možného dopadu na proteinovou strukturu, regulaci signálních drah apod. Výsledky dizertační práce přispějí k rozvoji personalizovaného přístupu k pacientům.

Interestingly, large amounts of gene mutations causing developmental abnormalities, e.g. orofacial diseases are shared with oncological diseases and they were found to be responsible for the induction of carcinogenesis. However, current knowledge of the associations of shared molecular characteristics between orofacial anomalies and carcinogenesis is insufficient. The goal will be to reveal the simultaneous occurrence of orofacial abnormalities and cancer and whether it would be possible to use the shared association of genes and found genetic variants, for example, for the targeted early detection of cancer. Methodologically, the dissertation will mainly deal with the preparation of exome and multigene sequencing libraries, possibly with other methods of analysis of genetic changes. In addition to early cancer screening, the dissertation will deal with interesting genomic changes that are detected during molecular profiling of tumors within the framework of routine predictive examinations. These are, for example, secondary reversion mutations, variants identified in the Kozak sequence, variants in the regions of exon splicing, etc. However, these aberrations are usually not studied and described more deeply, as it is beyond the scope of current clinical use. The genetic changes found will be described in detail in terms of their possible impact on the protein structure, regulation of signaling pathways, etc. The results of the dissertation will contribute to the development of a personalized approach to patients.

Návaznost na projekt AZV ČR, reg. č. NW24-10-00204.
Notes

Téma je rezervováno. This topic is reserved.

Supervisor

RNDr. Ing. Ondřej Bonczek, Ph.D.

Single-cell transcriptomics-guided identification of novel molecular targets and prognostic markers in neuroblastoma
Supervisor: RNDr. Jan Škoda, Ph.D.

Neuroblastoma is the most common and highly lethal extracranial pediatric solid tumor, characterized by extensive intratumor and interpatient heterogeneity that dictates its clinical manifestation and complicates its management. Currently, about one in ten low- and intermediate-risk patients is misclassified and eventually dies from progressive disease, while available therapies still fail in half of the high-risk neuroblastoma cases. Neuroblastoma arises anywhere along the sympathetic nervous system, probably from neural crest cell (NCC)-derived sympathoadrenal progenitors. Recent single-cell transcriptomics studies of our collaborators shed new light on NCC differentiation and provided unprecedented insights into differentiation trajectories and cellular transition states during normal sympathoadrenal development. As suggested by our published and preliminary results, translating this updated understanding of the sympathoadrenal development into neuroblastoma research might hold the key for unraveling neuroblastoma heterogeneity. The main aim of this thesis is to explore the utility of single-cell transcriptomics data for identification of clinically useful therapeutic targets and markers that could be streamlined into improved neuroblastoma treatment protocols.
Supported by the Ministry of Health of the Czech Republic, project NW24-07-00017.

Notes

PLEASE NOTE: Before initiating the formal application process to doctoral studies, interested candidates are required to contact Dr. Jan Škoda for informal discussion.

Supervisor

RNDr. Jan Škoda, Ph.D.

Supervisors

Study information

Provided by Faculty of Science
Type of studies Doctoral
Mode full-time Yes
combined Yes
distance No
Study options single-subject studies Yes
single-subject studies with specialization No
major/minor studies No
Standard length of studies 4 years
Language of instruction Czech
Collaborating institutions
  • The Czech Academy of Sciences
  • Biofyzikální ústav AV ČR
Doctoral board and doctoral committees

Do you have any questions?
Send us an e-mail to

doc. Mgr. Petr Beneš, Ph.D.

Consultant

E‑mail:

You are running an old browser version. We recommend updating your browser to its latest version.

More info