Publication details

Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability

Authors

KUNOVÁ Michaela MATULKA Kamil EISELLEOVÁ Lívia SALYKIN Anton KUBÍKOVÁ Iva KYRYLENKO Sergiy HAMPL Aleš DVOŘÁK Petr

Year of publication 2013
Type Article in Periodical
Magazine / Source STEM CELLS Translational Medicine
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.5966/sctm.2012-0081
Field Genetics and molecular biology
Keywords Embryonic stem cells; Cell culture; Xenograft assay; Cell survival; Induced pluripotent stem cells
Description The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high and gradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as a single-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system is highly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies.
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