Publication details

Mesalamine modulates intercellular adhesion intercellular adhesion through inhibition of p-21 activated kinase-1

Authors

KHARE V. LYAKHOVICH A. DAMMANN K. LANG M. BORGMANN M. TICHÝ Boris POSPÍŠILOVÁ Šárka LUCIANI G. CAMPREGHER Ch. EVSTATIEV R. PFLUEGER M. HUNDSBERGER H. GASCHE Ch.

Year of publication 2013
Type Article in Periodical
Magazine / Source Biochemical Pharmacology
MU Faculty or unit

Central European Institute of Technology

Citation
Doi http://dx.doi.org/10.1016/j.bcp.2012.10.026
Field Oncology and hematology
Keywords PAK1; mesalamine; beta-catenin; E-cadherin; cell adhesion
Attached files
Description Mesalamine (5-ASA) is widely used for the treatment of ulcerative colitis, a remitting condition characterized by chronic inflammation of the colon. Knowledge about the molecular and cellular targets of 5-ASA is limited and a clear understanding of its activity in intestinal homeostasis and interference with neoplastic progression is lacking. We sought to identify molecular pathways interfered by 5-ASA, using CRC cell lines with different genetic background. Microarray was performed for gene expression profile of 5-ASA-treated and untreated cells (HCT116 and HT29). Filtering and analysis of data identified three oncogenic pathways interfered by 5-ASA: MAPK/ERK pathway, cell adhesion and beta-catenin/Wnt signaling. PAK1 emerged as a consensus target of 5-ASA, orchestrating these pathways. We further investigated the effect of 5-ASA on cell adhesion. 5-ASA increased cell adhesion which was measured by cell adhesion assay and transcellular-resistance measurement. Moreover, 5-ASA treatment restored membranous expression of adhesion molecules E-cadherin and beta-catenin. Role of PAK1 as a mediator of mesalamine activity was validated in vitro and in vivo. Inhibition of PAK1 by RNA interference also increased cell adhesion. PAK1 expression was elevated in APC(min) polyps and 5-ASA treatment reduced its expression. Our data demonstrates novel pharmacological mechanism of mesalamine in modulation of cell adhesion and role of PAK1 in APC(min) polyposis. We propose that inhibition of PAK1 expression by 5-ASA can impede with neoplastic progression in colorectal carcinogenesis. The mechanism of PAK1 inhibition and induction of membranous translocation of adhesion proteins by 5-ASA might be independent of its known anti-inflammatory action.

You are running an old browser version. We recommend updating your browser to its latest version.

More info